Generator

Part:BBa_K4442001:Design

Designed by: Hala Mahdi   Group: iGEM22_McMaster_Canada   (2022-10-09)


Butyrate Sensing System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 734
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 75


Design Notes

According to the study authors: " I will caution that this sensor took us a lot of work to be functional. In particular, we had to delete the h-ns gene (a global regulator) to see a substantial change with butyrate. There is definitely a lot of opportunity for parts matching to tune the sensitive and dynamic range of the sensor.


Source

The pchA promoter, pchA, and LEE1 promoter sequences were all obtained from the original study authors, Yanfen Bai and Thomas J. Mansell.

References